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center : Isfahan University of Medical Sciences
Document Type : Latin Dissertation
Language of Document : English
Record Number : 102552
Doc. No : T8938
Call number : ‭WI,149,H348e,2003‬
Main Entry : Hasheminia, Javad
Title & Author : The eveluation of serum soluble ICAM- 1 and NK cytotoxicity in patients with cancer of colon and stomach
College : Schools, Medical
Date : , 2003=۱۳۸۳
Degree : Immunology, M.S
Page No : 126 p.: illus, tab
Note : This thesis is also a research project with project ID 81135
Abstract : Introduction: Cancer is an old dangerous disease in human being. One of the most common cancer in man in many countries is gastric cancer specially stomach and colon cancers. The highest annual incidence rates world wide in men are in Nagasaki (82 per 100,000) and Miyagi Japan (80 per 100,000). Most of the researches in cancer field have been done is the role of immune system against tumor cells. NK cells act against tumor cells in the body. This work is a study of measurement and comparison of serum soluble ICAM1 and NK cell binding and cytotoxicity in patients with stomach and colon cancers against normal controls and investigation for the effect of high levels of sICAM-1 on NK cells activity. also, there is a comparison between levels of sICAM-1 and CA19.9 tumor marker.Material and methods: The patients samples were obtained from Alzahra hospital and the control samples were taken from blood bank healthy donors and a privet section. The pathologic report was essential for cases selection. sICAM-1 kit was purchased from Roche company and the conjugated monoclonal antibodies were purchased from IQ Products Company for flow cytometryests. The K562 cell line was purchased from Cell Bank of PastorInstitute in Tehran. K562 cell line was cultured in RPMI+10 FCSand applied as target cells .The measurement of serum sICAM-1 and .....
Descriptor : 1- Gastrointestinal Neoplasms.- Descriptor(s): Flow Cytometry
: Killer Cells, Natural
: Cytotoxicity tests, Immunologic
: Cell adhesion Molecules
: Cytological techniques
: Colonic Neoplasms
: Stomach Neoplasms
: Intercellular Adhesion Molecule
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The eveluation of serum soluble ICAM- 1 and NK cytotoxici...
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