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" Evaluation of occult HBV infection in HBsAg negative and Anti_HBc positive blood donors "
/محمد کاظمی عربآبادی
; Abasali Poorazar, Mansor Salehi, Abdellah Jafarzadeh
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Isfahan University of Medical Sciences
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| Document Type
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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102568
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| Doc. No
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T9446
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WC,536,K23e,2005
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| Main Entry
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Kazemi Arababady, Mohammad
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| Title & Author
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Evaluation of occult HBV infection in HBsAg negative and Anti_HBc positive blood donors/محمد کاظمی عربآبادی
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| College
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Schools, Medical
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| Date
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, 2005
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| Degree
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Immunology, M.S
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| Page No
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xi, 117p.: ill; map.
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| Note
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Orginal works
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| Abstract
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Introduction: The risk of infection with transfusion-transmitted viruses has been reduced remarkably. However, a zero-risk blood supply remains a popular goal. Some authorities have introduced the screening for antibody to HBc (anti-HBc) as a surrogate test for the presence of several infectious agents. The aim of this study was to evaluate the prevalence of HBV infection markers and the potential value of anti-HBc testing of donors to detect occult HBV infection.Materials and methods: In this descriptive cross-sectional study, between April and Jun 2004, 545 blood units were collected and tested by HBsAg ELISA kit, then all HBsAg negative samples were tested by Anti-I-Mc ELISA kit. To detect occult HBV infection all HBsAg negative and Anti-HBc positive samples were tasted by PCR.Results: All samples were HBsAg negative but 43 blood units were Anti-I-Mc positive (8 ). These blood units were tested for HBV DNA by PCR. Five blood units were HBV DNA positive.Discussion: Occult HBV infection is a clinical form of HBV infection that HBsAg is not express by HBV and blood units cannot be screened by usual method (ELISA), therefore more sensitive techniques are needed. PCR is a sensitive technique that detects HBV DNA even in a small mount. Our results clearly demonstrated that more sensitive tests and complementary tests such as PCR and AntiHBc, respectively, are essential to ensure safety of blood units..
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| Descriptor
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1.Hepatitis B.- Descriptors: Hepatitis B Vaccines
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Blood Donors
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Infection
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Hepatitis B Antigens
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Blood Transfusion
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| Added Entry
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Poorazar, A, Supervisor
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Salehi, M, .Supervisor
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Jafarzade, A, Supervisor
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| Translated Title Supplied by Cataloguer
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بررسی عفونت نهفته هپاتیت B در افراد HbSAg منفی
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