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" Evaluation Of CD 11b and CD64 markers in neonatal sepsis for rapid diagnosis and compare with blood culture and serum CRP level "
/وجیهه استادی
; M. Adib, F. Navaei, F. Saheb Fosoul
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Isfahan University of Medical Sciences
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| Document Type
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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102578
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| Doc. No
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T9651
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| Call number
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WC,240,O85e
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| Main Entry
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Ostadi, Vajiheh
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| Title & Author
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Evaluation Of CD 11b and CD64 markers in neonatal sepsis for rapid diagnosis and compare with blood culture and serum CRP level/وجیهه استادی
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| College
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Schools, Medical
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| Date
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, [2005]
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| Degree
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Immunology, MS
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| Page No
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72 p.: ill., diag., tab
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| Note
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This thesis is also a research project with project ID 82193
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Orginal Works
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| Abstract
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Introduction: Neonatal sepsis is a life-threatening disease With an incidence of I to 10 per 1000 live births and a mortality rate of 15 to 50 . The clinical signs are nonspecific and indistinguishable from those caused by a variety of neonatal noninfective disorders. The aim of this study was to determine sensitivity, specificity, positive and negative predictive value of CD1 lb and CD64, two neutrophil markers, in early diagnosis of neonatal sepsis.Methods: In this study were comprised 65 neonates with a gestational age of 27 to 38 weeks who suspected for sepsis within 28 days of life. 1 cc of whole blood was obtained from neonates to determine of CD1 lb and CD64 expression on peripheral blood neutrophils by flow cytometry. CRP was measurement qualitatively. Neonates were classified into two groups. Classification was based on positive blood culture. In the sepsis group (n = 8), all of neonates were positive blood culture and had clinical symptoms. In the suspected group (n 57), each neonate had at least 1 clinical sign but negative blood culture. 12 healthy term neonates with physiologic hyperbilirubinemia classifed in control group. Results: Neutrophil CD 1 lb was elevated in sepsis and suspected group but this increase was not significant between three groups (P = 0.69). CD64 was elevated in sepsis group and this increase was significant (P<0.001)). CRP in 4 neonates with sepsis was positive and other neonates were negative.Sensitivity and specificity of CD1 lb and CD64 were 75 , 100 , 100 and 92.3 respectively. The negative and positive predictive value of CD1 lb and CD64 for identifying sepsis were 86 , 100 , 100 and 88 respectively.Discussion: Sensitivity of CD64 was higher than CD1 lb in neonatal sepsis. Although, specificity of CD1 lb was higher than CD64. Combining the use of CD64 with CD1 lb further enhances the ability to diagnosis infection and improve the sensitivity and negative predictive value to 100 ..
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| Descriptor
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1. Sepsis- diagnosis.- Descritors: C- Reactive Protein
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Antigens, CD11b
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Receptors, IgG
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Cytotoxicity Tests, Immunologic
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Neutrophils
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Serologic Tests
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Hyperbilirubinemia
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Flow Cytometry
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Sepsis Syndrome
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Cytodiagnosis
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| Added Entry
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Adib,M, .Supervior
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Navaei,F, .Supervisor
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Saheb Fosoul, F, .Superviser
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