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" Comparison Evaluation of Infants Fetal Erythrocytes with Haemolytic Disease of Newborn ( HDN ), in Maternal Blood Using Two Flow Cytometric Methods "
/ویدا همایونی
; Abas ali Pourazar, Abas Rezaei
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Isfahan University of Medical Sciences
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| Document Type
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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102580
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| Doc. No
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T9650
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| Call number
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WH,425,H763c,2005
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| Main Entry
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Homayouni, Vida
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| Title & Author
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Comparison Evaluation of Infants Fetal Erythrocytes with Haemolytic Disease of Newborn ( HDN ), in Maternal Blood Using Two Flow Cytometric Methods/ویدا همایونی
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| College
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Schools, Medical
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| Date
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, 2005
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| Degree
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Immunology, M.S
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70 p.: ill., Tab., diag.
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| Note
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This Thesis is also a research project with project ID: 82170
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| Abstract
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summary: Introduction: The quatification of fetal cells in the maternal circulation remains and imortant goal to determine the amount of anti-D positive baby. for this purpose, many centers use flow cytometry now. However chich flow cytomentric method is the most accurate is unknown. We estimated fetomaternal haemorrhage (FMH) by both directly conjugated anti-D and anti-fetal haemoglobin(HbF) labeling. Method: 10 samples of adult d negative blood were spiked wiht four serial dilution amounts of D+cord blood (0.25,0.5,1,2 fetal cells per cell)representing artificial FMH were established and then werestained by two methods (anti-D and anti-HbF staining) and were prepared for flow cytometry. Result: Two methods gave satisfactory results when performed with care. the result were correlate wiht expected percentage of fetal cells, FMH and measured percentage of fetal cells, FMH by flow cytometric methods (r2=0.9672,r2=0.8917). A very close correlation but significant difference was found between two methods. (P=0.002). Discussion: Our data showed that anti-D labelling significantly is much more accurate than HbF labelling. Quantification of FMH using these two techniques described allows precise dosage of RhD immunoglobulin for protection against anti-D allo-immunization. Hey Word: Fetomaternal Haemorrhage (FMH) / anti-D/Anti-HbF.
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Introduction: Low back pain is a very common patient complaint. Treatment of low back pain due to disc rupture is non-surgical in most cases. However, when conservative treatment fails to produce useful results, surgical treatment becomes mandatory. This study aimed to compart-the recurrence of pain in patients following discectomy with two different methosd, i.e. nucleotomy and subtotal discectomy.Methods and Material: A total of 73 patients were studied. The case and control groups comprised 36 and 37 patients respectively. Patients in the control group were treated with the usual method of discectomy (subtotal discectomy), and those in the case group underwent nucleotomy.Results: After a follow-up period of one year, 26 patients in the control group had developed low back pain, 17 of whom experienced pain relief following injection of contrast media and lidocaine into the affected joint. Only 7 patients in the case group had developed low back pain in the same period, 2 of whom experienced pain relief following injection of contrast media and lidocaine into the affected joint. Recurrence of pain was significantly lower in the case group (P <0.5)).Conclusion: Findings of this study suggest that the higher recurrence of low back pain in patients undergoing subtotal discectomy is due to decreased inervertebral space following surgery, which in trun leads to tension in the apophseal joint and subsequent development of synovitis.Introduction: Low back pain is a very common complaint of patients presening to primary care physicians and specialists(1).Every year, almost 200,000 patients undergo lumbar discectomy in Unied States(2). Based on existing guidenlines, surgery should be considered for patients with objective evidence of radiculopathy who have not responded to conservative treatment (3-5). Surgery is thought to improve symptoms by reliveing nerve roots of tension, pressure and resultant inflammation, however, the final decision about resorting to discectomy, as well as the outcome of this procedure depend on several factors inculding chronicity of disease, type of neurosurgical treatment and the patients' expectations of treatmnt(6-8). Failure of conservative treatment mandates lumbar discectomy(9-10), which can be performed with different methods..
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| Descriptor
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1. Erythroblastosis, Fetal.- Descriptors: Fetomaternal Transfusion
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Erythroblastosis, Fetal
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Immunity, Maternally- Acquired
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Immunization
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Flow cytometry
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Cytometry- Methods
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| Added Entry
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Pourazar, Abas Ali, Supervisor
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Rezaei, Abas, Supervisor
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| Translated Title Supplied by Cataloguer
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بررسی مقایسهای حضور اریتروسیتهای جنینی نوزادان مبتلا به ...
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