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Isfahan University of Medical Sciences
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| Document Type
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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102623
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| Doc. No
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T10281
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| Call number
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WE,500,M245s,2006
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| Main Entry
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Maleki, Farshid
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| Title & Author
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A STUDY OF MYOBLAST BEHAVIOUR IN VIVO AND IN VITRO. Farshid Maleki/فرشید مالکی
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| College
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Schools, Medical
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| Date
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, 2006
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| Degree
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Medicine, MD
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| Page No
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105 p.: ill ( col ), diag., tab
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| Note
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This thesis is performed in ROYAL COLLAGE OF SURGEONS IN IRELAND and then is presented for ( MD) in Isfahan medical university
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| Note
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Orginal Works
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| Abstract
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This project looked at prenatal myogenic cell orientation and response to surface features both in vivo and in vitro. This was done in order to understand how these cells are arranged prior to fusing into new skeletal musck fibre during myogenesis.In order to examine cell position in vivo, serial ultra thin sections spaced at 2 microns interval were cut through the mid belly of the Extensor Digitorum !engin muscle. This was done at 3 ages, embryonic day IA 17, E 19 and at birth. Electron micrographs of an individual fascicle were made for 20 planes over a distance of 40 microns. These were used to create computer based three dimensional images of cells of all the fibres and cells pre-sera in the. fascicles. The results showed 0.4t secondary fibres form between E 17 and birth. Cells identified by their appearance as myogenic cells are found on the surface of both primary and secondary fibres, All myogenic cells had their long axis parallel to the surrounding fibres. These cells appeared to be distributed randomly with respect to surface features around the perimeter of fibres at E 17, but at E 19 and birth cells are LOCALed mainly in grooves between adjacent primary and secondary fibres. At these ages there. is a significant correlation bemeen groove size and the number of cells present within a groove. Cell lotion within grooves and cell alignment parallel to existing fibres may help in the formation of new fibres. The alignment of cells was. also examined in vitro. The behaviour of myogenic cells from 3 different ages was also examined in vitro by culturing them on grooved quartz which had depth varying between 0.25 6.0 microns and width varying between 2.0 - microns. Myoblasts were found to respond to groove surfaces with a depth greater than I - 7 microns and a width between 2 - 25 microns by aligning parallel to the grooves. Cells from E 17 appeared to require deeper (2 microns) grooves to produce a response and overall depth was more significant than width in inducing cell alignment. These results may indicate that a mechanical response of myogenic cells to grooves enables them to orientate and align in rows prior to fusion into new fibres..
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| Descriptor
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1. Myoblasts.- Descriptors: Myoblasts
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Muscle Cells
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Muscle Development
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Cells, Cultured
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| Added Entry
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Wigmore, Peter, Supervisor
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http://elib.mui.ac.ir/site/catalogue/102623
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