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" Comparison between ARG( ABL-2)Protein expression follicular and Diffuse Large B-Cell lymphoma "
/زهرا کبیری
; Mansoor Salehi
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Isfahan University of Medical Sciences
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| Document Type
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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102666
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| Doc. No
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T10972
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| Call number
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WH,525,K11c,2007
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| Main Entry
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Kabiri, Zahra
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| Title & Author
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Comparison between ARG( ABL-2)Protein expression follicular and Diffuse Large B-Cell lymphoma/زهرا کبیری
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| College
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Schools, Medical
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| Date
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, 2007
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| Degree
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Medicine, MD
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| Page No
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64 p.: diag
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| Note
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This thesis is a also a research project with project ID: 83051
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| Note
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Orginal Works
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| Abstract
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Introduction: The participation of ARG in human malignancies has been demonstrated in different cancers. However there is no conclusive evidence on ARG expression between different mature B- cell lymphomas. In this study we evaluated ARG protein expression in FL, DLBCL, and non-neoplastic lymphoid tissue and also we compared expression of ARG protein between these mature B cell lymphomas. Materials and methods: Twelve DLBCL patients and twelve FL patients with available Paraffin-embedded tissue samples between 1997 and 2004 enter the study, we used 12 reactive lymph nodes as control samples. Sections were prepared from the paraffin blocks and treat with primary and then secondary antibodies. Two proteins, ARG (the test protein) and GAPDH (a control house keeping protein) were studied; ARG was labeled green and GAPDH red. After capturing the pictures using a CCD camera, the intensity of green and red colures were measured and ratio between green/red (ARG/GAPDH), that demonstrate changes in ARG expression, were calculated. Results: we detected the ARG and GAPDH expression in tissue samples of DLBCL, FL patients and reactive lymph nodes. The mean ratio of ARG/GAPDH expression in DLBCL and FL was 0.742 and 0.688 respectively. Also this ratio in reactive lymph node was 0.651. The P value between our groups was less than 0.001 so it can clearly show significant different between groups. Conclusion: Like other malignancies, finding about gene expression patterns in BL seems to be helpful in future board therapeutic guidelines. Our result showed that aggressive lymphoma (DLBCL) has higher expression of ARG compare to Indolent lymphoma (FL). Gleevec (ST571) as a specific inhibitor of ARG may have a potential potency to treat of aggressive lymphoma.
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| Descriptor
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1.Lymphoma.- Descriptors: Lymphoma- drug therapy
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Lymphoma B-Cell
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Gene Expression
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Lymph Noeds
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Immunohistochemistry
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Proteins
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Lymphoma, Folicular
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| Added Entry
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Salehi, Mansoor, Supervisor
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| Translated Title Supplied by Cataloguer
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مقایسه بیان پروتئین ARG در لنفوم فولبکولار و لنفوم سلولهای B بزرگ منتشر
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