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" A Study of synaptic transmission of dorsal root afferents on motoneurons of isolated a dult mouse spinal cord "
/مهرنوش مقدسی
; Hojjatallah Alaei, Mohammad Reza Sharifi, Ali Nasimi
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Isfahan University of Medical Sciences
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| Document Type
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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102669
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| Doc. No
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T10968
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| Call number
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WL,102.8,M696a,2007
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| Main Entry
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Moghaddasi, Mehrnoush
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| Title & Author
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A Study of synaptic transmission of dorsal root afferents on motoneurons of isolated a dult mouse spinal cord/مهرنوش مقدسی
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| College
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Schools, Medical
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| Date
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, 2007
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| Degree
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Physiology, Ph.D
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| Page No
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110 p.:( some col ), diag
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| Note
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This thesis is also a research project wiht project ID: 385464
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| Note
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Orginal Works
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| Abstract
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Spinal cord motoneurons appear to be unusually vulnerable to cell injury and death, which has hampered their study in situ. Understanding of the mechanism of their death and injury is important in relation to a variety of diseases, including spinal cord injury.Recently, a number of isolated mammalian spinal cord preparations for studying these cells have been developed, but most of these have involved developmentally immature animals. This developmental immaturity limits the use of these preparations to study the neurophysiology of the spinal cord including synaptic transmission.The present study describes a viable preparation of isolated spinal cord of mature mice, removed from the vertebral canal by hydraulic extrusion technique. This is the first time that hydraulic extrusion .has been employed in the study motoneurons. With this technique, the spinal cord is extruded and isolated much faster than with conventional laminectomy. The isolated spinal cord preparation was characterized from different aspects using morphological, histological and electrophysiological techniques. The preparation shows segmental synaptic responses recorded extracellularly from the ventral horn of lumbosacral segments, which are stable for up 5-6 hours in vitro. The synaptically mediated nature of recorded motoneuronal response was confirmed by their sensitivity to the application of glutamate receptor blockers Kynurenic acid (1mM) or NBQX (10)1M), which reversibly suppressed the response (p<0.01). The responses were unaffected by NMDA receptor blocker AP5 (501..tM). This indicates that the monosynaptic activation of motoneurons occurs predominantly via non-NMDA receptors. The motoneuron responses after application of GABA receptor blocker, bicuculline, didn't change significantly but after the combined application of strychnine and bicuculline, the antagonists of glycine and GABA respectively, significantly decreased (p<0.05), further studies will provide more information about this inhibitory pathway. The antidromic population spikes exhibited evident paired pulse inhibition when evoked at inter-stimulus intervals of < 6 msec. Histological examination revealed that basic morphological features of the lumbo-sacral motoneurons were preserved after 3-4 h of in vitro maintenance. This in vitro preparation seems well suited for the electrophysiological study of synaptic transmission on adult mouse spinal motoneurons..
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| Descriptor
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1.Reseptors, Neurotransmitter.- Descriptors: Spinal Cord Injuries
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Motor Neurons, Gamma
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Synapses
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Receptors, N-Methyl- D-Aspartate
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Glycine
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Laminectomy
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Receptors, Neurotransmitter
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| Added Entry
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Alaei, Hojjatallah, Supervisor
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Sharifi, Mohammad Reza, Supervisor
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Nasimi, Ali, Supervisor
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| Translated Title Supplied by Cataloguer
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ارزیابی ارتباطات سیناپسی نورونهای آوران حسی ریشه خلفی در نورونهای حرکتی نخاع ایزوله موش سوری بالغ
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