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" Phytochemical and Biological Studies of some Marines from Iran and Thailand "
/افسانه یگدانه
; Alireza Ghannadi, Anuchit Plubrukara
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Isfahan University of Medical Sciences
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| Document Type
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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103292
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| Doc. No
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T14503
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QV,766,Y43p,2013
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| Main Entry
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Yegdaneh, Afsaneh
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| Title & Author
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Phytochemical and Biological Studies of some Marines from Iran and Thailand/افسانه یگدانه
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| College
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Schools, Pharmacy
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| Date
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, 2013
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| Degree
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Pharmacognosy, Ph.D
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| Page No
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122 p.: ill., diag., tab
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| Note
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This thesis is a research project with project ID 389176
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| Note
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Orginal Works
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| Abstract
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Marine compounds are among the most important natural products. In this study, the phytochemical investigation of 8 seaweeds, Cystoseira indica, C. merica, Sargassum angostifolium, S. oligocystum, S. boveanum, Padina australis, Gracilaria corticata and G. salicornia was performed. Besides, antimalarial, atituberculosis, acetyl choline esterase inhibitor and cytotoxic activities of them were evaluated. The sponge Myrmekioderma spp. As well as a soft coral from Thailand were phytochemicaly analyzed and their pure compounds were elucidated. Marine organisms were collected by diving, dried and powdered. MeOH: EtOAC (1:1) extracts of seaweeds were subjected to antituberclusis, antimalaria, ACEI and cytotoxic tests by Microculture radioisotope technique, GFPMA, microplate assay methods and Sulphorodamin. Seaweeds S. angostifolium and S. oligocystom were partitioned in solvents with different polarities and subjected to cytotoxic tests. All marines were extracted and chromatographed using Flash chromatography, MPLC and HPLC. Interested fractions were selected based on preliminary 1H-NMR analysis and TLC profile in each step. Isolated compounds were elucidated using 1H-NMR, 13C-NMR, 2D NMR (1H-1HCOSY, HMBC, HMQC), IR and MS spectra. At last antiproliferative activity of the isolated compounds from seaweeds and sponge were analyzed. From C.indica, a cyclic depsipeptide with new skeleton, fatty acids such as palmitic and oleic acid and steroids including fucosterol and 24-ethylcholest-5-en-3-ol were isolated. The depsipeptide showed cytotoxic activity on HeLa cell line (IC50=0.286+- 0.018). Six pure compounds were isolated from the sponge Myrmekioderma sp., Out of which, there were Three new sesquiterpenes, 10-Oxoabolene, 12-oxocurcuphenol and 10-hydroxyabolene, together with curcuphenol, curcodiol and Indole-3-carbaldehyde. The antiproliferative activity of these compounds determined to show an interesting selectivity; i.e., a remarkable susceptibility in HT-29 cells. Five cembrane diterpenes, (+) sarcophine along with its hydroxyl derivatives were isolated from soft coral. It was the first time that 9a-hydroxysarcophine, 9B-hydroxysarcophine, 10B -hydroxysarcophine and 10a- hydroxysarcophine were isolated from nature. Isolating new compounds with significant structures from investigated marines besides their biological activities from one side and rich sources of marines in our country from the other side, indicates the necessity of the marines. Full biological and phytochemical analyzing will lead to find newer compounds with better activity.
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| Descriptor
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1. Plants, Medicinal.- Descriptors: Plants, Medicinal- terapeutic use
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Seaweed
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Chromatography
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Phytochemicals
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| Added Entry
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Ghannadi, Alireza, Supervisor
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Plubrukara, Anuchit, Supervisor
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| Translated Title Supplied by Cataloguer
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بررسی فیتوشیمیایی و بیولوژیک برخی از موجودات دریایی ایران و تایلند
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