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" Comparison of the immunosuppressive effects of silymarin with rapamycin and FK506 on the proliferation and apoptosis of T cells in vitro "
/احسان الماسی
; Nahid Eskandari, Marjan Gharagozloo
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Isfahan University of Medical Sciences
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| Document Type
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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103513
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| Doc. No
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T15159
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QW,920,A446c,2014
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| Main Entry
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Almasi, Ehsan
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| Title & Author
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Comparison of the immunosuppressive effects of silymarin with rapamycin and FK506 on the proliferation and apoptosis of T cells in vitro/احسان الماسی
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| College
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Schools, Medical
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| Date
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, 2014
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| Degree
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Immunology, M.A
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| Page No
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91 p.: tab., Diag
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| Note
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This thesis is a research project with project ID: 392573
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| Abstract
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silymarin, as an polyphenolic flavonoid derived from milk thistle (silybummarianum) is known to have antioxidant, immunomodulatory, antiproliferative, antifibrotic, and antiviral effects . the goal of this study was to determine immunosuppressive effect of silymarin on proliferation and apoptosis of human T cells in comparson with rapamycin and FK506. peripheral blood mononulcear cells (PBMCs) from the healthy individuals were activated with con a (5ug/ml)and then treated with silymarin, rapamycin and FK506 at various concentrations (0.001, 0.01, 0.1, 1, 10, 100 and 200 uM) for 5 days. PBMCs were examined for proliferation using the CFSE assay and the comcentration that inhibited 50 percent of the cell proliferation (IC 50) was determined for each treatment. for apoptosis assay using floww cytometry, PBMCs were activated with con A and treated with IC 50 dose of silymarin, rapamycin and FK506 for five days, then cell apoptosis was analyzed by FITC-annexin V/PI staining and flow cytometry. the effects of silymarin, rapamycin and FK506 on the activation of PARp (poly ADP ribose polymerase) pathway in PBMCs stimulated with con A and treated with IC50 dose of drugs for five days evaluated using the pathScan cleaved PARP sandwich ELISA kit. this study showed that silymarin had the ability to inhibit T cell proliferation in vitro.Moreover our results indicated that 100 uM and 200 uM of silymarin showed inhibitory effect on T cells proliferation cpmpared with FK506 and rapamycin. data showed that the inhibitory effect of silymarin, FK506 and rapamycin on the T cell proliferation was not due to cytotocicity and none of these drugs at the Ic50 concentration had no effect on the level of cleaved PARP. this study shows that silymarin has the ability to inhibit T cell proliferation in vitro and exert immunosuppressive effects. lilymarin could be a good candidate for immunosuppressive therapy for certain medical conditions with superior efficacy and lesser toxicity compared with other immunosuppressive drugs.
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| Descriptor
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1. Immunosuppressive Agents.- Descriptors: Immunosuppressive Agents
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Silymarin
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Sirolimus
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T-Lymphocytes
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Tacrolimus
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| Added Entry
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Eskandari, Nahid, Supervisor
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Gharagozloo, Marjan, Supervisor
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| Translated Title Supplied by Cataloguer
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بررسی اثر ایمونوساپرسیو سیلیمارین با FK506 و راپامایسین بر تکثیر و آپوپتوز لنفوسیتهای T فعال شده انسانی در شرایط in vitro
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