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" Production and characterization of monoclonal antibodies against Human Her2 and evaluation of its effect on breast cancer cell line SK-BR-3 "
/منیژه مهدوی
; Abbas Jafarian Dehkordi, Mohammad Rabbani, Mehrnaz Keyhanfar
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Isfahan University of Medical Sciences
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| Document Type
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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103540
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| Doc. No
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T15331
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QW,525,M214p,2014
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| Main Entry
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Mahdavi, Manijeh
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| Title & Author
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Production and characterization of monoclonal antibodies against Human Her2 and evaluation of its effect on breast cancer cell line SK-BR-3/منیژه مهدوی
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| College
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Schools, Pharmacy
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| Date
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, 2014
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| Degree
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Pharmaceutical Biotechnology, Ph.D
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XVii, 152p.: ill, diag, tab
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| Note
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This thesis is a research project with project ID 190152
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| Abstract
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Introduction: Worldwide, breast cancer is a major public health problem. Although, in Iran cancer is the third cause of death after coronary heart disease and accidents, it`s mortality is on the rise during recent decades. About 15 percent to 20 percent of patients with invasive breast cancer have abnormally high levels of the HER2 protein. HER2 is a specialized protein found on breast cancer cells that controls cancer growth and spread. methods: This study describes generation and characterization of new anti-HER2 mAbs towards HER2 protein using a chimeric peptide immunogen containing discontinuous B-cell epitope peptide (peptide 626) and promiscuous T-helper epitope (MVF). The chimeric peptide was designed by bioinformatics analysis. MAbs were isotyped using isotyping kit. They characterized by SDS-PAGE, Western blotting, and immunofluorescence. The effects of purified mAbs on breast cancer (SK-BR-3) cell proliferation were evaluated by MTT assay. Results: We generated three IgG isotype monoclonal antibodies (1A11, 5H5 and 5H11) and seven IgM isotype mAbs using a standard hybridoma technology. The specificity of these mAbs was confirmed by various immunoassays, including ELISA, Western blotting, and immunofluorescence. In addition, the MTT assay results indicated that 5H5 and 5H11 mAbs could reduce growth of SKBR3 cells by approximately 50 (P < 0.05). Discussion: These mAbs that can reduce cancer cells proliferation would be useful for cancer therapy. Furthermore, the synthetic peptide used in the current work, was able to induce immune system to generate antibodies especially IgG isotype. Therefore, it could be further used as cancer peptide vaccine that target different epitope or structural domain of HER2 ECD..
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| Descriptor
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Antibodies, Monoclonal
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Breast Neoplasms
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Receptor, ErbB-2
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Jafarian Dehkordi, Abbas, Supervisor
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Rabbani, Mohammad, Supervisor
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Keyhanfar, Mehrnaz, Supervisor
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Chou, Perry, Supervisor
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| Translated Title Supplied by Cataloguer
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تولید و تعیین خصوصیات آنتی بادی های مونوکلونال بر ضد رسپتور HER2 انسانی و بررسی اثرات آن بر رده سلول های سرطانی سینه SK-BR-3
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