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" Comparison of the frequencies of TIM-3 4259 AC and TIM-1 -1637 CT polymorphisms in the Multiple Sclerosis patients and healthy controls "
/Masoumeh Pouladian
; Mazdak Ganjalikhani Hakemi, Rasoul Salehi
; Fereshteh Alsahebfosul
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Isfahan University of Medical Sciences
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| Document Type
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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104741
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| Doc. No
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T15548
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| Call number
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QU500,P784c,2015
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| Main Entry
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Pouladian, Masoumeh
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| Title & Author
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Comparison of the frequencies of TIM-3 4259 AC and TIM-1 -1637 CT polymorphisms in the Multiple Sclerosis patients and healthy controls/Masoumeh Pouladian
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| College
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Schools, Medical
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| Date
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, 2015
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| Degree
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Immunology, M.S
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| Page No
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60 p.: ill, tab
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| Note
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This thesis is a research dissertation with project ID 392267
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معصومه پولادیان
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| Abstract
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Introduction: Multiple sclerosis (MS) is an inflammatory disease of the central nervous system (CNS) which initiated and mediated by autoreactive T helper1 cells directed against myelin antigens. One of T cell surface receptors is T cell immunoglobulin and mucin domain (TIM) family. There are several single nucleotide polymorphisms (SNPs) in their sequences witch had associated with susceptibility to different autoimmune diseases. The aim of this study was to investigate the susceptibility of MS patients in Isfahan population with polymorphisms +4259 A>C in TIM-3 and -1637 C>T in TIM-1 gene. Material and methods: Blood samples were collected. DNA was extracted from the blood samples using Genomic DNA Extraction Kit. Polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) was performed. TIM-3 and TIM-1 genes were amplified using PCR. Then PCR products were digested with restriction enzymes, PstI and RsaI. Electrophoresis was performed for separating the digested products. Result: bout 4259 A>C in TIM-3, genotype and allele carrier frequency between patient and healthy groups were statistically significant (P = 0.029). Odds of susceptibility to MS for whom carrying C allele of TIM-3 compared with those who do not carry, was about 2 (p= 0.01). for -1637 C>T , T allele frequency in patients with MS (1.4 %) was more than healthy controls ( 0.0 ) but the Fisherʼs exact test showed no significant difference between the two groups ( P= 0.06 ). Conclusion: our findings suggests that +4259 A>C polymorphism in exon 3 of TIM-3 is associated with Multiple Sclerosis in Iranian population. In order to better understanding the role of TIM-3 and TIM-1, as well as their polymorphisms in MS, measuring the expression level of the genes carrying such polymorphisms and their relevant protein functional and/or structural analysis could be helpful
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| Descriptor
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Polymorphism, Genetic
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T-Lymphocytes
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Membrane Proteins
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Multiple Sclerosis
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| Added Entry
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Ganjalikhani Hakemi, Mazdak Thesis advisor
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Salehi , Rasoul, Thesis advisor
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Alsahebfosul, Fereshteh, Consulting advisor
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| Translated Title Supplied by Cataloguer
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مقایسه فراوانی پلی مورفیسم ژن TIM-3 در بیماران دچار مولتیپل اسکلروزیس و افراد سالم
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