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center : Isfahan University of Medical Sciences
Document Type : Latin Dissertation
Language of Document : English
Record Number : 104800
Doc. No : T15552
Call number : ‭QW504,H353c,2015
Main Entry : Hassanzadeh, Mohammad Ali
Title & Author : Comparison of the immunophenotyping of the peripheral blood transitional B cells and evaluation of the B cell activation factor of the TNF family receptor mutations in the patients with common variable immunodeficiency with the allergic or non-allergic symptoms and healthy controls/ Mohammad Ali Hassanzadeh
College : Schools, Medical
Date : , 2015
Degree : Immunology, M.A
Page No : 63 p.: ill, tab
Note : This thesis is a research dissertation with project ID 3922488
: محمد علی حسن زاده
Abstract : Introduction: Common variable immunodeficiency (CVID) is a heterogeneous group of the primary immunodeficiency disorders and the commonest primary immunodeficiency syndrome. CVID is accompanied by the hypogammaglobulinemia and recurrent bacterial infections This thesis aimed to determine the immunophenotyping of the peripheral blood transitional B cells and the B cell activation factor of the TNF family receptor (BAFFR) mutations in the patients with common variable Immune deficiency (CVID) with the allergic or non-allergic symptoms compared to the healthy controls.Methods: Genomic DNA was extracted from the whole blood of the patients with CVID and healthy controls (n=15) using an extraction kit (genet bio). Primers were designed to both sides of each exon. After confirming the accuracy of the PCR, the product was sent to the sequencing. The peripheral blood mononuclear cells (PBMC) were isolated by the Ficoll-Hypaque density centrifugation. For the transitional B-cell phenotype, samples were washed in phosphate-buffered saline before staining. The CD19-FITC (Exebio), CD24-PE (Exebio), and CD38 PE (BD Exebio) antibodies were used.The staining process was performed according to the manufacturers’ protocol and then the cells were run on a FACS Caliber, BD. The data was analyzed using Cell Quest software.Results: The results of the sequence and alignment of the sequences, did not show mutations in the gene .The flow cytometric indicated that the mean percentages of B cells in the allergic patients group (3.68 ± 0.96, p<0.001) and non-allergic patients group (1.2 ± 0.57, p <0.001) were significantly different from the controls (10.43 ± 3.46).
Descriptor : Common Variable Immunodeficiency
: Immunophenotyping
: Peripheral Blood Stem Cell Transplantation
: Agammaglobulinemia
: Mutation
Added Entry : Eskandari , Nahid, Thesis advisor
: Ganjalikhani Hakemi, Mazdak Thesis advisor
: Sherkat, Roya, Thesis advisor
: Khan Ahmad, Hossein, Consulting advisor
Translated Title Supplied by Cataloguer : بررسی مقایسه ای فنوتیپ های B ترانژیشنال و جهش ژنی در پذیرنده فاکتور فعال کننده سلول B در بیماران دچار نقص ایمنی رایج متغیر با علائم بالینی آلرژیک با افراد نرمال
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Comparison of the immunophenotyping of the peripheral blo...
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