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" Prevalence rate of acquired carbapenemase genes in Klebsiella Pneumoniae by Multiplex PCR in samples that achieved from ICUs patients "
\ Aliasghar Soudi
; Mohammadreza Yazdani, Farzin Khorvash
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Isfahan University of Medical Sciences
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| Document Type
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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105041
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| Doc. No
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T15610
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WC260,S719p,2015
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| Main Entry
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Soudi, Aliasghar
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| Title & Author
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Prevalence rate of acquired carbapenemase genes in Klebsiella Pneumoniae by Multiplex PCR in samples that achieved from ICUs patients \ Aliasghar Soudi
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| College
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Schools, Medical
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| Date
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, 2015
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| Degree
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Infectious, Speciality
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| Page No
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14 p.: tab
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| Note
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This is a research dissertation with project ID:392127
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علی اصغر سودی
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| Abstract
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Background: Multi drug resistant Klebsiella pneumoniae has been considered a serious global threat. This study was done to investigate carbapenemase producing genomes among K. pneumoniae isolates in Isfahan, central Iran.Methods: in a cross sectional study From 2011 to 2012, 29 carbapenem resistant (according to disc diffusion method) carbapenemase producing (according to modified Hodge test) K. pneumoniae strains were collected from ICUs of Al-Zahra referral hospital. In the strains with the lack of sensitivity to one or several Carbapenems, Beta- Lactams or Beta- Lactamases; there has been performed Modified Hodge Test to investigate Carbapenmase and then only strains producing Carbapenmases were selected for molecular methods. Results: In this study, there have been 29 cases of Klebsiella Pneumoniae isolated from hospitalized patients in the intensive care unit (ICU). Three cases (10.3%) contained blaVIM, 1 case (3.4%) contained blaIMP and 1 case (3.4%) contained blaOXA. The genes blaNDM and blaKPC were not detected. Then, 16 cases (55.2%) from positive cases of Klebsiella Pneumoniae were related to the chip, 4 cases (13.8%) to catheter, 6 cases (20.7%) to urine and 3 cases (10.3%) to wound.Conclusion: It is necessary to monitor the epidemiologic changes of these carbapenemase genes in K. pneumonia in our hospital. More attention should be paid to nosocomial infection control measures. Other carbapenemase-producing genes should be investigated
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| Descriptor
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Klebsiella pneumoniae
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Drug Resistance, Microbial
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beta-Lactamases
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Multiplex Polymerase Chain Reaction
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Genes
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Yazdani, Mohammadreza, Thesis advisor
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Khorvash, Farzin Thesis advisor
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| Translated Title Supplied by Cataloguer
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میزان شیوع ژن کارباپنماز به دست آمده از کلبسیلا پنومونیه به روش Multiplex PCR در نمونه های بایگانی شده بیماران بخش مراقبت ویژه
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