| |
| center
|
:
|
Isfahan University of Medical Sciences
|
| Document Type
|
:
|
Latin Dissertation
|
| Language of Document
|
:
|
English
|
| Record Number
|
:
|
106468
|
| Doc. No
|
:
|
T16503
|
| Call number
|
:
|
QU55.3,O55c,2015
|
| Main Entry
|
:
|
Omidi Najafabadi, Elham
|
| Title & Author
|
:
|
The Cytotoxic Effect Of Memantine And It's Effect On Cytoskletal Proteins Expression In Metastatic Breast Cancer Cell Line\ Elham Omidi Najafabadi
|
| College
|
:
|
Schools, Medical
|
| Date
|
:
|
, 2015
|
| Degree
|
:
|
Medicine
|
| field of study
|
:
|
M.D
|
| Page No
|
:
|
9p.: diag
|
| Note
|
:
|
This is a research dissertation with project ID: 394454
|
|
|
:
|
الهام امیدی نجف آبادی
|
| Abstract
|
:
|
Breast cancer is the leading cause of death from cancer. Stathmin and tau protein are regulators of cell motility and overexpression of them in breast cancer is associated to the progression and bad prognosis of the disease. Memantine, an N-methyl-D-aspartate (NMDA) receptor antagonist, is the potential inhibitor of tau protein in neurons.This study determines the effect of memantine on breast cancer cell migration and proliferation, tau and stathmin gene expression in cancer cells and its synergistic effect with paclitaxel.The cell proliferation was evaluated by MTT assay and for this purpose, MCF-7 breast cancer cells were treated with various concentration of memantine (2, 20 and 100 μg/ml). Tau and stathmin mRNA expression was evaluated through quantitative realtime RT-PCR method. The migration of cancer cells treated with memantine for 24 hours was compared to no treated cells using an in vitro transmembrane migration assay.Incubation of breast cancer cells with memantine resulted in a dose dependent reduction in cell survival (P=0.0001).100 nM concentration of paclitaxel showed synergistic effect with memantine(P=0.0001).Memantine significantly decreased tau and stathmin mRNA expression(by RT-PCR), dose dependently,100µmol/L 46%(p=0.0341)decreased tau expression and 33% (p=0.043) decreased stathmin expression. Migration of cells was also decreased by memantine (p=0.0001)Memantine can be used as a potential adjuvant chemotherapeutic agent in combination with paclitaxel.Tau and stathmine proteins might have a prominent role in breast cancer metastasis.
|
| Descriptor
|
:
|
Cytoskeletal Proteins
|
|
|
:
|
Gene Expression
|
|
|
:
|
Memantine
|
|
|
:
|
Breast Neoplasms
|
|
|
:
|
Cell Line
|
| Added Entry
|
:
|
Haghjooy Javanmard, Shaghayegh, Thesis advisor
|
| Translated Title Supplied by Cataloguer
|
:
|
اثر سیتوتوکسیتی ممانتین و اثر آن بر روی بیان پروتئین اسکلت استخوان در دست اندازی خط سلولی سرطان پستان
|
| | | | | |
| | |  |
http://elib.mui.ac.ir/site/catalogue/106468
| | | |
| | | |
|