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" Effect of DAPT, a gamma secretase inhibitor, on tumor angiogenesis in control mice "
\ Elmira Kalantari
; Majid Khazaei
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Isfahan University of Medical Sciences
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| Document Type
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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106850
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| Doc. No
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T16494
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| Call number
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QU107,K14e,2015
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| Main Entry
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Kalantari, Elmira
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| Title & Author
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Effect of DAPT, a gamma secretase inhibitor, on tumor angiogenesis in control mice\ Elmira Kalantari
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| College
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Schools, Medical
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| Date
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, 2015
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| Degree
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M.D
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| field of study
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Medicine
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| Page No
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4p.: ill, diag, tab
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| Note
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This is a research dissertation with project ID: 190036
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المیرا کلانتری
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| Abstract
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Background: Notch signaling is a key factor for angiogenesis in physiological and pathological condition and γ‑secretase is the regulator of Notch signaling. The main goal of this study was to assess the effect of (N‑[N‑(3,5‑Diflurophenaacetyl‑L‑alanyl)]‑S‑phenylglycine t‑Butyl Ester) DAPT, a γ‑secretase inhibitor, on serum angiogenic biomarkers, and tumor angiogenesis in control mice.Materials and Methods: Tumor was induced by inoculation of colon adenocarcinoma cells (CT26) in 12 male Balb/C mice. When tumors size is reached to a 350 ± 50 mm3, the animals were randomly divided into two groups: control and DAPT (n = 6/group). DAPT was injected subcutaneously 10 mg/kg/day. After 14 days, blood samples were taken and the tumors were harvested for immunohistochemical staining.Results: Administration of DAPT significantly increased serum nitric oxide concentration and reduced vascular endothelial growth factor receptors‑1 (VEGFR1) concentration without changes on serum VEGF concentration. DAPT reduced tumor vascular density in control mice (280.6 ± 81 vs. 386 ± 59.9 CD31 positive cells/mm2), although, it was not statistically significant.Conclusion: It seems that γ‑secretase inhibitors can be considered for treatment of disorders with abnormal angiogenesis such as tumor angiogenesis
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| Descriptor
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Angiogenesis Inhibitors
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Amyloid Precursor Protein Secretases
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Adenocarcinoma
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Obesity
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| Added Entry
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Khazaei, Majid, Thesis advisor
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| Translated Title Supplied by Cataloguer
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اثر دی ای پی تی یک گاماسکرتاز اینهیبیتور بر دانسیته مویرگی در آدنوکارسینوم کولون القا شده در موش های نرمال
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