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" Gamma-secretase inhibitor does not modulate angiogenesis in colon adenocarcinoma in obese mice "
\ Hajar Saeidi
; Bahman Rashidi
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Isfahan University of Medical Sciences
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| Document Type
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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107452
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| Doc. No
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T17071
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QU107,S127g,2016
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| Main Entry
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Saeidi, Hajar
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| Title & Author
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Gamma-secretase inhibitor does not modulate angiogenesis in colon adenocarcinoma in obese mice\ Hajar Saeidi
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| College
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Schools, Medical
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| Date
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, 2016
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| Degree
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M.D
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| field of study
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Medicine
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{No paging}: ill, diag
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| Note
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This is a research dissertation with project ID: 190038
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هاجر سعیدی
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| Abstract
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Abstract: Background: Notch is a signaling molecule which plays a role in angiogenesis and γ-secretase isrequired for processing of Notch. In this study, we investigated the effect of γ-secretase inhibitor (DAPT) ontumor angiogenesis in diet-induced obese mice.Methods: 18 mice were divided into three groups; control, obese (diet-induced) and obese+DAPT. After 15 weeks,the obese mice were subjected for tumor induction of CT26 colon adenocarcinoma cells (5 x 105 cells). Whenthe tumor size reached approximately 350 ± 50 mm3, half of the obese animals received DAPT (10mg/kg/day)subcutaneously. Blood samples were taken after 14 days and the tumors harvested for immunohistochemicalstaining and capillary density were reported as CD31 positive cells/mm2.Results: The obese animals had higher serum leptin and NO concentrations, while, serum VEGF and VEGFR-1 concentrationswere not different compare to control group. Administration of DAPT in obese mice signifi cantly reduced serumVEGFR-1 and leptin concentrations and increased serum NO level (p < 0.05). Capillary density in the tumors of obeseanimals was not different compare to control groups. DAPT administration could not alter capillary density in the tumors.Conclusion: Administration of DAPT in obese mice altered serum angiogenic factors, however, it could not modulatetumor angiogenesis in diet-induced obese mice (Fig. 4, Ref. 26). Text in PDF www.elis.sk.Key words: γ-secretase, obese, angiogenesis, adenocarcinoma.
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| Descriptor
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Angiogenesis Inhibitors
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Amyloid Precursor Protein Secretases
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Colonic Neoplasms
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Adenocarcinoma
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Obesity
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Mice
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| Added Entry
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Rashidi , Bahman , Thesis advisor
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| Translated Title Supplied by Cataloguer
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مهارکننده گاما سکرتاز نمیتواند تنظیم کند آنژیوژنز را در آدنوکارسینومای روده در موش های چاق
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