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" Feasibility of genotyping of single nucleotide polymorphism rs8066560 of SREBF1c gene in metabolic syndrome subjects with tetra-primer ARMS PCR technique and its comparison with TaqMan Real-Time PCR method "
\ Hoda Miranzadeh Mahabad
; Parvaneh Nikpour
; Roya Kelishadi, Modjtaba Emadi Baygi
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Isfahan University of Medical Sciences
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| Document Type
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Latin Dissertation
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English
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| Record Number
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108001
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| Doc. No
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T17179
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| Call number
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WD210,M672f,2015
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| Main Entry
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Miranzadeh Mahabad, Hoda
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| Title & Author
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Feasibility of genotyping of single nucleotide polymorphism rs8066560 of SREBF1c gene in metabolic syndrome subjects with tetra-primer ARMS PCR technique and its comparison with TaqMan Real-Time PCR method\ Hoda Miranzadeh Mahabad
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| College
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Schools, Medical
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| Date
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, 2015
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| Degree
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M.D
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| field of study
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Medicine
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{No paging}: ill, diag, tab
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| Note
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This is a research dissertation with project ID: 293049
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هدی میران زاده مهابادی
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| Abstract
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Background. Single nucleotide polymorphisms (SNPs) are major contributors to susceptibility or resistance tovarious human diseases. Metabolic syndrome (MetS) is a collection of risk factors, including abdominal obesity,dyslipidemia and increased blood pressure. MetS is more common among Iranian children and adolescents incomparison to other ethnicities. Sterol regulatory element binding factors (SREBFs) involve in the regulation ofcarbohydrate and lipid metabolism. The tetra-primer amplification refractory mutation system PCR (tetra-primerARMS-PCR/T-ARMS-PCR) is a fast and economical means of assaying SNPs, requiring only PCR amplificationand subsequent electrophoresis for the determination of genotypes.Objectives. This study aims to optimize and compare the results of tetra-primer ARMS-PCR for genotyping ofrs8066560 in Iranian children and adolescents being afflicted with metabolic syndrome with the TaqMan assay.Material and Methods. In this study, a total of 50 individuals with 9–19 years of age, including 25 healthy subjectsand 25 MetS cases were studied. The tetra-primer ARMS-PCR was used to genotype the rs8066560.Results. After PCR optimization, we could successfully detect the rs8066560 polymorphism in all the studiedsubjects. Furthermore, we observed complete concordance between tetra-primer ARMS-PCR assay and TaqManmethod`s results.Conclusions. Tetra-primer ARMS-PCR can be utilized as a cost-effective, rapid and reproducible method forSNP genotyping especially while performing large-scale epidemiological/association at studies (Adv Clin Exp Med2015, 24, 6, 000–000).
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| Descriptor
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Metabolic Syndrome X
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Polymorphism, Genetic
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Gene Expression
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Child
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Adolescent
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Polymerase Chain Reaction
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Genotype
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Nikpour, Parvaneh, Thesis advisor
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Kelishadi, Roya, Consulting advisor
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Emadi Baygi, Mojtaba, Consulting advisor
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| Translated Title Supplied by Cataloguer
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امکان سنجی تعیین ژنوتیپ پلی مورفیسم تک نوکلئوتیدی در افراد مبتلا به سندروم متابولیک
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