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" Comparing the efficacy of routine H&E staining and cytokeratin immunohistochemical staining in detection of micro-metastasis on serial sections of dye-mapped sentinel lymph nodes in colorectal carcinoma "
/ Mohammad Hossein Sanei
; Abbas Tabatabie, Mozafar Hashemi, Ali Cherei, Parvin Mahzouni, Behnam Sanei
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English
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طرح تحقیقاتی/ پروژه لاتین
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| Record number
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109286
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R3265
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| main entry
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Sanei, , Mohammad Hossein
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| title & author
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Comparing the efficacy of routine HE staining and cytokeratin immunohistochemical staining in detection of micro-metastasis on serial sections of dye-mapped sentinel lymph nodes in colorectal carcinoma [research project]\ / Mohammad Hossein Sanei; Abbas Tabatabie, Mozafar Hashemi, Ali Cherei, Parvin Mahzouni, Behnam Sanei
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| Publication statement
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Isfahan: , Esfahan University of Medical Sciences, Vice Chancellery for Research, 2011
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| Physical Description
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[ No Paging ]: .: tab.
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| Notes
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Project ID: 184156
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| Summary or Abstract
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Background:The significance of techniques used for detecting micro-metastasis (MM) or isolated tumor cells (ITCs) is a controversial issue among investigators. We evaluated the different techniques used on sentinel lymph node (SLN) to detect MM/ITCs.Materials and Methods:Ninety-one SLNs of 15 patients underwent serial section with 100 μm interval. In each level, two sections were prepared. One section was stained with H&E and another with anti-cytokeratin antibody (immunohistochemistry). Then the sections were evaluated for detecting MM/ITCs. Results were analyzed by chi-square test.Results:1656 sections of 91 SLNs of 15 patients were evaluated by a pathologist; MM was found in 1 and ITCs in 1 case. Overall, 2 out of 15 cases (13.3% of the patients) showed MM/ITCs by IHC staining. So, serial section along with using IHC was superior than serial section and routine H&E staining. But it did not affect the 5-year survival of the patients (P = 0.47).Conclusion:Using the combined techniques of serial section and IHC staining could up-stage 13.3% of colon cancer patients who were lymph node negative. In other studies with different combination of serial section, IHC staining, and PCR, investigators were able to find MM/ITCs in 3-39% of the cases. In our study, although serial section and IHC staining could up-stage 13.3% of patients, it could not affect the 5-year survival of the patients.
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Colorectal Neoplasms
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Sentinel Lymph Node Biopsy
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Immunohistochemistry
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Lymphatic Metastasis
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| added entry
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Tabatabie, Abbas, Cllaborator
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Hashemi, Mozafar, Cllaborator
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Mahzoni, Parvin, Cllaborator
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Sanei, Behnam, Cllaborator
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Cherei , Ali, Cllaborator
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| Originating Source
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IRIsfahan University of Medical Science
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p
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