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" Molecular typing of Brucella species isolates from Human and livestock bloods in Isfahan province "
/ Ebtehaj Pishva
; Akbar Hoseini, Ali Kargar, , Rasoul Salehi, Fatemeh Emani Taba, Maryam Hajiyan, Reza Fadaei, Javad Ramezanpour
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English
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| Material Type
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طرح تحقیقاتی/ پروژه لاتین
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| Record number
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109306
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| doc. No
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R3252
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| main entry
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Pishva, , Ebtehaj
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| title & author
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Molecular typing of Brucella species isolates from Human and livestock bloods in Isfahan province [research project]\ / Ebtehaj Pishva; Akbar Hoseini, Ali Kargar, , Rasoul Salehi, Fatemeh Emani Taba, Maryam Hajiyan, Reza Fadaei, Javad Ramezanpour
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| Publication statement
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Isfahan: , Esfahan University of Medical Sciences, Vice Chancellery for Research, 2014
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| Physical Description
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5 p: .: tab.
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Project ID: 185234
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| Contents
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عنوان به فارسی: ژنوتایپینگ انواع بروسلا در استان اصفهان با روش PCR_ RFLP
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| Summary or Abstract
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Background:Human brucellosis is caused by infection with certain species of the genus Brucella and is characterized by bacterial persistence and inflammation of many host tissues. Handling all live Brucella involves risk of laboratory infection and very strict biosafety rules must be observed. In order to avoid these disadvantages, method based on the PCR-RFLP shows excellent typeability, reproducibility, stability, and epidemiological concordance. The omp2 locus contains two gene copies (named omp2a and omp2b) coding for porin proteins and has been found particularly useful for molecular typing and identification of Brucella at the species, biovar, or strain level. This study is designed to evaluate the molecular epidemiology of Brucella spp from human and livestock in Isfahan province, central region of Iran in order to use the findings in efficient disease prevention programs.Materials and Methods:One hundred ninety blood samples were collected from human and cattle with active brucellosis and 40 aborted ewes fetuses were collected and genotyped using PCR-RFLP technique, DNA polymorphisms such as the restriction patterns of the PCR-amplified omp2a and omp2b genes.Results:The molecular characterization performed to assess the species and the biovar of the Brucella strains. Analysis of the 230 isolates examined in this study generated three unique RFLP profiles. One of the profiles was the most common being present in 134/180.Conclusion:Our findings confirm abundance of B. melitensis, particularly biovar 1 in human and sheep are identical but B. abortus biovar 3 as the etiological agent of cattle brucellosis most frequently isolated in the Isfahan area.
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Brucella
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Brucellosis
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Molecular Typing
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Polymerase Chain Reaction
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Livestock
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| added entry
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Hoseini, Akbar, Cllaborator
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Kargar, Ali, Cllaborator
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Salehi, Rasoul, Cllaborator
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Emani Taba , Fatemeh, Cllaborator
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Hajiyan , Maryam, Cllaborator
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Fadaei , Reza , Cllaborator
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Ramezanpour , Javad, Cllaborator
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| Originating Source
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IRIsfahan University of Medical Science
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| publication type
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p
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