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" Evaluation of the prophylaxis effects of Derp2 vaccine with adjuvants (MPLA, CPG, BCG) on acute atopic asthma in Balb/c mice "
\ Vahid Mohammadi Shahrokhi
; Nahid Eskandari
; Abdollah Jafarzadeh
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Isfahan University of Medical Sciences
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| Document Type
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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109357
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| Doc. No
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T17315
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| Call number
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QW800,M697e,2016
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| Main Entry
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Mohammadi Shahrokhi, Vahid
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Eskandari , Nahid, Thesis advisor
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| Title & Author
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Evaluation of the prophylaxis effects of Derp2 vaccine with adjuvants (MPLA, CPG, BCG) on acute atopic asthma in Balb/c mice\ Vahid Mohammadi Shahrokhi
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| College
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Schools, Medical
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| Date
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, 2016
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| Degree
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Ph.D
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| field of study
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Immunology
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| Page No
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xii, 93p.: ill, diag, tab
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| Note
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This is a research dissertation with project ID: 393816
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وحید محمدی شاهرخی
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| Abstract
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Background and Aims: The house dust mite (HDM) is the most inducer of allergies including asthma which consists of several components specially allergens Derp1 and Derp2.The impairment of T helper (Th1), Th2 and T regulatory (Treg) cells play key roles in asthma pathogenesis. Toll like receptors (TLR) agonists such as the monophosphoryl lipid A (MPLA), CpG oligodeoxynucleotides (CPG-ODN) and bacillus Calmette–Guérin (BCG) are the main adjuvants with their effects on the induction of T cells. The aim of the present study was to evaluate the CPG, MPLA and BCG effects on Th1- Th2 and Treg -related parameters on an asthmatic BALB/c mice model. Materials and Methods: Five groups of BALB/c mice were immunized on days 1, 15, and 22 with Derp2. Three groups pre-treated on days 0, 14, and 21 with CPG, CPG+MPLA or CPG+BCG. Challenge performed with Derp2 on days 28-37. The saline- or Derp2-sensitized control group received saline or Derp2, respectively. Blood samples obtained on days 0, 23 and 40. The serum IL-4, IFN-γ, IgE and IgG2a levels were measured using ELISA. Blood Th1 and Treg cell frequency were detected using flow cytometry. Pathological studies were performed on lungs and bronchoalveolar lavage fluid (BALF) using standard methods. Results: At the sensitization phase, the, IFN-γ and IgG2a levels, IFN-γ/IL4, IgG2a/IgE and Th1/Treg ratios in CPG plus MPLA group, Treg cell frequency in CPG or CPG plus MPLA groups, IFN-γ/IL4 ratio in groups of CPG, CPG plus MPLA or CPG plus BCG were significantly higher than Derp2-sensitized control mice (P<0.05). At the challenge phase, the IFN-γ/IL-4,Th1 cells frequency in groups CPG plus MPLA, CPG plus BCG or CPG, Treg cells frequency and Th1/Treg ratios in CPG plus BCG group, IFN-γ levels, IgG2a in CPG plus MPLA group and Th1/Treg ratio in CPG plus MPLA group were significantly higher than Derp2-sensitized control mice. The Th1/Treg ratio in CPG plus MPLA group was also higher than CPG plus BCG group. The Th1/Treg ratio in CPG plus BCG group was lower than CPG group. The lung inflammatory scores in groups of CPG, BCG and CPG plus MPLA were lower than Derp2-control group (P<0.05). The BALF levels of eosinophils and total cells in Derp2-sensitized group pre-treated with CPG, CPG plus MPLA and CPG plus BCG, were significantly lower than in Derp2-sensitized control group (P≤0.001). Conclusion: Derp2 vaccine plus BCG, MPLA and CPG modulate Th1- Th2- and Treg-related parameters, improve their imbalances and ameliorate inflammation in a mouse asthma model.
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| Descriptor
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Interferon-gamma
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Asthma
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Immunoglobulins
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Interleukin-4
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| Added Entry
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Jafarzadeh , Abdollah, Consulting advisor
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| Translated Title Supplied by Cataloguer
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ارزیابی تاثیر پیشگیری واکسن حاوی آلرژن Derp2 به همراه ادجوانت های BCG,MPLA,CPG بر روی آسم اتوپیک حاد در موش Bslb/c
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