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" Investigation of erythrocyte membrane fatty acid profile and Na+/K+-ATPase activity and their association with insulin resistance and cardiovascular risk factors in type 2 diabetic men with and without metabolic syndrome "
Fouzieh Zadhoush
Morteza Pourfarzam
Masoumeh Sadeghi
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Isfahan University of Medical Science
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| Document Type
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Latin Dissertation
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| Language of Document
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English
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| Record Number
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111263
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| Doc. No
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T17842
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QU 90,Z17i,2016
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| Main Entry
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Zadhoush, , Fouzieh
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| Title & Author
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Investigation of erythrocyte membrane fatty acid profile and Na+/K+-ATPase activity and their association with insulin resistance and cardiovascular risk factors in type 2 diabetic men with and without metabolic syndrome\ Fouzieh Zadhoush
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| College
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School of Pharmacy and Pharmaceutical Sciences
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2016
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Ph.D
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| field of study
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Clinical Biochemistry
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173p.
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| Note
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Research project Number: 393087
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| Abstract
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Introduction:The pathophysiology of the link between type 2 diabetes mellitus (T2DM) and cardiovascular disease (CVD) is complex and multifactorial. Understanding the mechanisms of this association can help clinicians to identify and treat patients at high CVD risk, as well as help them prevent potentially devastating complications. To achieve this goal, we investigated the erythrocyte membrane fatty acids (EMFAs) profile and Na+/K+-ATPase activity and their association with insulin resistance and cardiovascular risk factors in T2DM patients with and without metabolic syndrome (MetS). Identifying the causative relationship between EMFAs composition and biochemical changes in T2DM men, fundamentally contributes to the understanding of the basic pathophysiological mechanisms of the disease and its complications.Materials and methods:In this cross-sectional study, 155 men between 40 and 60 years of age were recruited. Subjects were distributed into three groups, namely Controls: including 50 normoglycemic healthy subjects; Group I: 50 T2DM without MetS and no apparent sings of complication; and Group II: 55 T2DM with MetS. After recording age, anthropometric factors and blood pressure, fasting blood samples was collected for measurement of blood glucose, insulin, HbA1c, high sensitivity C-reactive protein (hs-CRP) and lipid profile. TG/HDL-C ratio, Atherogenic index of plasma and homeostatic model assessment of β-cell function and insulin resistance (HOMA-B and HOMA-IR) were calculated. Erythrocyte membrane protein content, total and Na+/K+-ATPase activity, the erythrocytes membrane fatty acid composition and their associations with other blood parameters were determined.Results:There were significant differences in biochemical and anthropometric parameters, between the studied groups. There was significant decrease in erythrocyte membranes Na+/K+-ATPase activity in T2DM patients compared to Controls, but no significant difference was observed in Na+/K+-ATPase activity between diabetic patients and in total ATPase activity in all subjects. There were significantly differences in the EMFAs profile between groups. There were significant increased in saturated fatty acids (TSFAs) and significant decreased in mono unsaturated fatty acids (MUFAs) and polyunsaturated fatty acids (PUFAs, 20:3n-6, 20:4n–6, 22:4n–6, 22:5n–3) and unsaturation index (UI)] in Group I compared to Controls. There were significant decreased in C22:4n–6 and C22:5-n3 and highly decreased in PUFAs, TPUFAs/TSFAs and UI in Group II compared to Controls. There were relationships between EMFAs and Na+/K+-ATPase activity, the individual components of MetS, fasting serum insulin, Hb A1c, HOMA-IR and hs-CRP levels in all subjects and/or in each group. There were relationships between Na+/K+-ATPase activity and HOMA-IR with some of the components of MetS.Discussion and conclusion:Given the above observations, unfavorable changes were occurred in EMFAs composition and Na+/K+-ATPase activity, serum lipid profile and other biochemical parameters, marker of inflammation and anthropometric indices in T2DM patients compared to Controls. Such alterations in membranes may have important effects on the structure and physicochemical properties of cell membranes, insulin secretion and its biological activities and membrane-bound enzymes. Such modifications of erythrocyte membrane composition may occur in cells participating in the cardiovascular regulation, thus might be considered as a part of pathogenic mechanisms responsible for the development of diabetes and its complications
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| Descriptor
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fatty acids
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erythrocyte Membrane
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Gas Chromatography-Mass Spectrometry
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insulin Resistance
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Type 2 Diabetes Mellitus
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metabolic Syndrome
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اسیدهای چرب
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غشاء اریتروسیت
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سندروم متابولیک
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Pourfarzam , Morteza
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Sadeghi , Masoumeh
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Isfahan University of Medical Sciences
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| Translated Title Supplied by Cataloguer
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بررسی پروفایل اسیدهای چرب و فعالیت آنزیم Na+/K+-ATPase غشاء اریتروسیت و ارتباط آنها با مقاومت به انسولین و ریسک فاکتورهای قلبی عروقی در مردان دیابتی نوع 2 با و بدون سندرم متابولیک
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