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language of document : English
Material Type : طرح تحقیقاتی/ پروژه لاتین
Record number : 51485
doc. No : R3046
main entry : Golzar, Fatemeh
title & author : The Effect of Kisspeptin- 10 on Migration of Mesenchymal Stem Cells [Research Project]/Executer: Fatemeh Golzar; ETC: Shaghayegh Haghjooy Javanmard
Publication statement : Isfahan: Isfahan University of Medical Sciences, Vice Chancellery for Research, 2013.
Physical Description : 10 p.:diag., tab
Notes : عنوان به فارسی : بررسی اثر کیسپپتین-‭ ۱۰ ‬بر روی میزان مهاجرت سلولهای بنیادی مزانشیمی به سمت تومور در موشهای‭ C57BL6‬مبتلا به تومور ملانوما
Notes : Kisspeptins (Kp) activate a receptor coupled to Gaq subunits (GPR54 or KiSS-1R) receptor to perform a variety of functions including inhibition of cell motility, chemotaxis and metastasis. In this study we have investigated whether kp-10, the most potent member of kisspeptin family, can modulate CXCR4 (C-X-C chemokine receptor type 4) expression and migration of mesenchymal stem cells (MSCs) that may influence the development of tumor. We compared the directional migration of MSCs treated with or without 10-100-500 nM kisspeptin-10 for 24 hours using an in vitro trans membrane migration assay. Chloromethylbenzamido Dialkylacarbocyanine (CM-Dil) labeled Adipose derived mesenchymal stem cells treated with or without 10-100-500 nM kisspeptin-10 were transfused to melanoma tumor bearing C57BL/6 mice. After 24 hr mice were scarified and tumors were dissected and tumor cell suspensions were analysed by flow cytometry for detection of CM-Dil +MSCs. We found that kp-10 increased MSCs migration at 100 nM while decrease MSCs migration at 500 nM both in vitro and in vivo with no significant effect on CXCR4 expression in different concentrations. Thus, our data showed that kp-10 can differently affect migration of MSCs in different concentrations, not through CXCR4 expression dependent mechanism.
Notes : Print
descriptor : Chemotaxis
: Kisspeptins
: Mesenchymal Stromal Cells
Originating Source : IRIsfahan University of Medical Sciences
publication type : p
Source : Vice Chancellery for Research
Ended Date : 2013
Project code : 190095
 
 
 
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