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" Evaluatingthe effect of varying ephrin/B2-FC concintrations on EPHB4 receptor phosphorylation and quantifying receptor level at sub and post confluent STATES BY IMMUNOASSAY IN MDA-MB-231 BREAST CANCER CELL LINE "
/Executer: Mir Ali Mohammad Sabzghabaei, Hamid Mir Mohammad Sadeghi
; ETC: Farnaz Barneh, Mona Mashidi, Fatemeh Moazen, Shahin Shafizadeghan
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English
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| Material Type
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طرح تحقیقاتی/ پروژه لاتین
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| Record number
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51502
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| doc. No
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R3159
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| main entry
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Sabzghabei, Mir Ali Mohammad
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| title & author
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Evaluatingthe effect of varying ephrin/B2-FC concintrations on EPHB4 receptor phosphorylation and quantifying receptor level at sub and post confluent STATES BY IMMUNOASSAY IN MDA-MB-231 BREAST CANCER CELL LINE [Research Project]/Executer: Mir Ali Mohammad Sabzghabaei, Hamid Mir Mohammad Sadeghi; ETC: Farnaz Barneh, Mona Mashidi, Fatemeh Moazen, Shahin Shafizadeghan
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| Publication statement
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Isfahan: Isfahan University of Medical Sciences, Vice Chancellery for Research, 2012.
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| Physical Description
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[No Paging ].:diag, tab
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| Notes
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عنوان به فارسی : بررسی اثر تغییرات غلظت لیگاند2Ephrin - Fc بر فسفریلاسیون گیرنده 4EphB و تعیین مقدار گیرنده در حالت هایSub - ConfluentوPost - Confluentدر رده سلولیMDA-MB- 231
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EphB4 is a tyrosine kinase receptor which exhibits different expression levels and activity in normal vs. tumor cells. Studies have shown that up-regulation of EphB4 concomitant with decreased phosphorylation promotes growth and invasion of tumor cells but the precise mechanism of tumor promotion by this receptor is not fully understood yet. Thus the aim of our study was to detect EphB4 receptor level at different states of confluency and its phosphorylation status in MDA-MB-231 breast cancer cell line following various ephrinB2-Fc concentration. To do so, cells growing in sub and post-confluent states were lysed and protein concentration was determined. Cell lysates were added to pre-coated wells and optical density was measured. For phosphorylation assay, serum-starved cells were stimulated with various concentrations of pre-clustered ephrinB2 for 20 minutes and were lysed to be detected. Expression of EphB4 receptor was detected to be higher in post-confluent state than sub-confluent. Phosphorylation status was detectable with by ELISA method following exogenous stimulation of cells and extent of phosphorylation was proportional to ligand concentration. Based on these results, EphB4 receptor is potentially active in tumor cells and. Moreover, it was shown ELISA method is rapid and sensitive enough to detect even low levels of total and phosphorylated EphB4 in this cell line thus the test can be performed with consuming less reagents.
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| Notes
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Print
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| descriptor
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Receptor, EphB4
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Protein Kinases
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Breast Neoplasms
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Antineoplastic Surgery
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| Originating Source
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IRIsfahan University of Medical Sciences
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p
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| Source
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Vice Chancellery for Research
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| Ended Date
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2012
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| Project code
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187148
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